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prehybridisation solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prehybridisation solution
    Prehybridisation Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prehybridisation+solution/SSC+buffer/pmc02362003-68-50-72
    Average 99 stars, based on 1 article reviews
    prehybridisation solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Diurnal Fluctuations in Steady-State mRNAs of Certain Chloroplast-Encoded Photosynthesis-Related Genes in Rice
    Article Snippet: Both light intensity and light quality vary during the day.. As the day progresses, the component of direct radiation vis-a-vis diffuse radiation declines commonly leading to increase in blue light accompanied by a marginal decrease in R/FR ratio (1).. As the plant responds to these variations in light quality and quantity, the transcript levels for components of the photosynthetic apparatus are also expected to change to help maintain their ratio for optimal light absorption and maintenance of high quantum efficiency (2).

    Hybridization:

    Article Title: Diurnal Fluctuations in Steady-State mRNAs of Certain Chloroplast-Encoded Photosynthesis-Related Genes in Rice
    Article Snippet: Both light intensity and light quality vary during the day.. As the day progresses, the component of direct radiation vis-a-vis diffuse radiation declines commonly leading to increase in blue light accompanied by a marginal decrease in R/FR ratio (1).. As the plant responds to these variations in light quality and quantity, the transcript levels for components of the photosynthetic apparatus are also expected to change to help maintain their ratio for optimal light absorption and maintenance of high quantum efficiency (2).

    Article Title: Insulin-like growth factor binding protein-6 inhibits prostate cancer cell proliferation: implication for anticancer effect of diethylstilbestrol in hormone refractory prostate cancer
    Article Snippet: The Cy3 or Cy5 labelled cDNA after purification was mixed, and 30 μ g of human Cot I DNA (Invitrogen), 16 μ g of poly-dA (Amersham Phamacia) and 20 μ g of yeast tRNA (Sigma) were added to reduce nonspecific binding. .. The probes were resuspended in 10 μ l of hybridisation solution consisting of 6 × SSC, 0.2% SDS and 5 × Denhardt's solution (Invitrogen), and incubated at 98°C for 2 min and then 4°C for 10 s. An IntelliGeneTM Human Cancer CHIP Version 2.1 (Takara) glass slide was treated with prehybridisation solution consisting of 6 × SSC, 0.2% SDS, 5 × Denhardt's solution and 1 mg ml −1 denatured salmon sperm DNA (Invitrogen). ..

    Article Title: Measurement of locus copy number by hybridisation with amplifiable probes
    Article Snippet: .. Filters were prehybridised together in 1 ml of prehybridisation solution (0.5 M sodium phosphate pH 7.2, 7% SDS, 0.1 mg/ml alkali-denatured herring sperm DNA) at 65°C, and before hybridisation this was exchanged for 200 µl of prehybridisation solution with the addition of denatured human Cot-1 DNA (Gibco BRL) to a final concentration of 10 µg/ml. .. Probe mixtures containing ~100 pg of each sequence in 1 µl were mixed with 7 µg Escherichia coli DNA (DH5α DNA, digested with Hae III), 0.5 µg ΦX174/ Hae III DNA, 20 pmol ‘end-blocking’ primers PZAX (AGTAACGGCCG-CCAGTGTGCTGGAATTCTGCAGAT) and PZBX (CGAG-CGGCCGCCAGTGTGATGGAT), and 1 µg human Cot-1 DNA (Gibco BRL), and denatured by adding 2 µl 1 M NaOH.

    Incubation:

    Article Title: Insulin-like growth factor binding protein-6 inhibits prostate cancer cell proliferation: implication for anticancer effect of diethylstilbestrol in hormone refractory prostate cancer
    Article Snippet: The Cy3 or Cy5 labelled cDNA after purification was mixed, and 30 μ g of human Cot I DNA (Invitrogen), 16 μ g of poly-dA (Amersham Phamacia) and 20 μ g of yeast tRNA (Sigma) were added to reduce nonspecific binding. .. The probes were resuspended in 10 μ l of hybridisation solution consisting of 6 × SSC, 0.2% SDS and 5 × Denhardt's solution (Invitrogen), and incubated at 98°C for 2 min and then 4°C for 10 s. An IntelliGeneTM Human Cancer CHIP Version 2.1 (Takara) glass slide was treated with prehybridisation solution consisting of 6 × SSC, 0.2% SDS, 5 × Denhardt's solution and 1 mg ml −1 denatured salmon sperm DNA (Invitrogen). ..

    Concentration Assay:

    Article Title: Measurement of locus copy number by hybridisation with amplifiable probes
    Article Snippet: .. Filters were prehybridised together in 1 ml of prehybridisation solution (0.5 M sodium phosphate pH 7.2, 7% SDS, 0.1 mg/ml alkali-denatured herring sperm DNA) at 65°C, and before hybridisation this was exchanged for 200 µl of prehybridisation solution with the addition of denatured human Cot-1 DNA (Gibco BRL) to a final concentration of 10 µg/ml. .. Probe mixtures containing ~100 pg of each sequence in 1 µl were mixed with 7 µg Escherichia coli DNA (DH5α DNA, digested with Hae III), 0.5 µg ΦX174/ Hae III DNA, 20 pmol ‘end-blocking’ primers PZAX (AGTAACGGCCG-CCAGTGTGCTGGAATTCTGCAGAT) and PZBX (CGAG-CGGCCGCCAGTGTGATGGAT), and 1 µg human Cot-1 DNA (Gibco BRL), and denatured by adding 2 µl 1 M NaOH.



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